Effect of Grape Seed Extract (vitis vinifera, sp) on Histopathology Appearance, CMYC and BCL-2 Gene Expression of AOM-DSS Induced Mice

Dimas Erlangga, Gaga Nugraha, Nurhalim Shahib


The use of Grape Seed Extract (GSE) as a dietary supplement to prevent cancer in Indonesia is increasing. The extract content such as resveratrol, quercetin, epigalochatecin and proanthocyanidin, are reported to increase apoptosis and prevents cancer cell proliferation which is shown by the expression level of cMyc and Bcl-2 gene. Azoxymethane (AOM) and Dextran sulfate sodium (DSS) are two chemical compounds used to induce cancer in mice. Methods: Fifteen female mice were divided into 3 groups, whose treatment was sequentially as follows: I. Induced by AOM- DSS; II. Induced by AOM-DSS and EBA; and III. Neither induced by AOM-DSS nor given EBA. Histopathology examination of the colons was presented in descriptive report. Analysis of c-Myc and Bcl-2 gene was performed; the gene expression level was determined using the Livak method and statistically analyzed using the Mann-Whitney test. Result: Dysplasia were shown in group I, mild dysplasia over the repair tissue in group II, and healthy tissue in group III. Using the Livak methods, cMyc and Bcl-2 gene expression of group II are 1.4 times stronger and 1.9 times stronger than group I. But statistically both of the result are non-significant (cMyc, P = 0.251; Bcl-2, P = 0.754). Discussion: Grape seed extract has no effect on the decrease in cMyc and Bcl-2 gene expression in AOM-DSS induced mice. The extract dose used has anti-inflammatory effect, which was shown on mice colon tissue histopathology but no anti cancer effect. To get the anticancer effect, it takes GSE with higher doses. In further research, it is necessary to use multiple doses of the GSE in order to know the effective dose to prevent the occurrence of colon cancer in mice induced AOM-DSS. 

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