The Combined Effect Of Omega-3 And Vitamin E As Neuroprotective Agent In Rats Induced Diazepam: Random Post Control Study Using Morris Maze Water

Surya Saptono, Yogi Haditya, Putri Ratnasari


In Indonesia, 2.3 billion benzodiazepines were consumed in a year and about 81% were diazepam

used. They used them for treating sleep lifecycle as sedative hypnotic that can cause damage on

neural system. The aim of this study is to know the effect of combination omega-3 and vitamin E on

cognitive and memory function in induced diazepam male rats. This study was a randomized post

test control group trial. First, 20 male wistar rats (weight 180-200 gram) kept under standardized

diet and condition for 7 days. On day 8th until 10th, all of they were trained in Morris maze water

for 10 minutes duration each day. On every training, that search hidden platforms and memorized it

for 20s. Escape Latency Time (ELT) was an index memory and cognitive. On day 11 until 20, they

given diazepam 1 mg via IM into a control positive group (I1), (I2), and (I3). Then the rats treated

with 1 soft gel for (I2), 2 soft gel for (I3) food supplement omega 3 and vitamin E via oral for 10

days. The result showed the escape latency time (ELT) between control group and treatment groups

was significantly different (p<0.05). The results indicate that combination omega-3 and vitamin E is

useful to enhance cognitive and memory function in induced diazepam male rats. 

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