Quaternized Cyclodextrin Grafted Chitosan Associated With High Molecular Weight Hyaluronic Acid Nanoparticles For Cosmetics

Sakhiran Sakulwech, Nattaya Lourith, Uracha Ruktanonchai, Sarunya Phunpee, Mayuree Kanlayavattanakul


An excellent moisturizer, hyaluronic acid (HA(, provides biocompatibility and viscoelastic properties that is extensively utilized in cosmetics. High molecular weight HA (HMW-HA, 1.2 MDa( is a typical used form, however its benefits is limited due to its large molecule. In our previous study, the association with quaternized cyclodextrin grafted chitosan (QCD-g-CS( can improve skin penetration and provide positive charge promoting skin adhesion. Thus, the purpose of this study was to produce and characterize HMW-HA associated QCD-g-CS nanoparticles for cosmetic utilization. The HMW-HA nanoparticles (0.01 HA per 1 QCD-g-CS mole ratio( was the best formula with low particles size (348.20 ± 8.67 nm(, narrow polydispersity index (0.083 ± 0.02(and positive zeta potential (16.00 ± 0.36 mV(. The association efficiency and loading efficiency were analyzed by UPLC and shown to be 82.64 ± 0.39% and 20.09 ± 0.57%, respectively. The conjugation between carboxylic moieties of HMW-HA and amide of QCD-g-CS was confirmed by FTIR. The TEM imaging showed the spherical morphology of these nanoparticles. According to cytotoxicity test, these nanoparticles (0.01 to 0.10 mg/ml( were safe in human skin fibroblasts.

Full Text:



Chen, M., Gupta, V., Anselmo, A. C., Muraski, J. A., & Mitragotri, S. (2014(. “Topical delivery of hyaluronic acid into skin using SPACE-peptide carriers.” J. Control. Release, 173(1), 67-74.

Cowman, M. K., & Matsuoka, S. (2005). “Experimental approaches to hyaluronan structure.” Carbohydr. Res., 340(5), 791-809.

Farwick, M., Lersch, P., & Strutz, G. (2008). “Low molecular weight hyaluronic acid: its effects on epidermal gene expression & skin ageing.” SÖFW J., 134(11), 2–6.

Gillet, A., Compère, P., Lecomte, F., Hubert, P., Ducat, E., Evrard, B., & Piel, G. (2011). “Liposome surface charge influence on skin penetration behaviour.” Int. J. Pharm., 411(1-2),223-231.

Gonil, P., Sajomsang, W., Ruktanonchai, U. R., Pimpha, N., Sramala, I., Nuchuchua, O., et al. (2011). “Novel quaternized chitosan containing β-cyclodextrin moiety: Synthesis, characterization and antimicrobial activity.” Carbohydr. Polym., 83(2), 905-913.

Kanlayavattanakul, M., Lourith, N., Tadtong, S., & Jongrungruangchok, S. (2015). “Rice panicles: New promising unconventional cereal product for health benefits.” J. Cereal Sci., 66(1), 10-17.

Kanlayavattanakul, M., Lourith, N., & Chaikul, P. (2016). “Jasmine rice panicle: A safe and efficient natural ingredient for skin aging treatments.” J. Ethnopharmacol., 193(1), 607-616.

Lademann, J., Richter, H., Teichmann, A., Otberg, N., Blume-Peytavi, U., Luengo, J., et al. (2007). “Nanoparticles–an efficient carrier for drug delivery into the hair follicles.” Eur. J. Pharm. Biopharm., 66(2), 159-164.

Phunpee, S., Saesoo, S., Sramala, I., Jarussophon, S., Sajomsang, W., Puttipipatkhajorn, S., et al. (2016). “A comparison of eugenol and menthol on encapsulation characteristics with watersoluble quaternized β-cyclodextrin grafted chitosan.” Int. J. Biol. Macromol., 84(1), 472-480.

Sajomsang, W., Gonil, P., Ruktanonchai, U. R., Pimpha, N., Sramala, I., Nuchuchua, O., et al. (2011). “Self-aggregates formation and mucoadhesive property of water-soluble β-cyclodextrin grafted with chitosan.” Int. J. Biol. Macromol., 48(4), 589-595.

Sakulwech, S., Lourith, N., Ruktanonchai, U., & Kanlayavattanakul, M. (2018). “Preparation and characterization of nanoparticles from quaternized cyclodextrin-grafted chitosan associated with hyaluronic acid for cosmetics.” Asian J. Pharm. Sci., 13(1), 498-504.


  • There are currently no refbacks.