Use Of Antagonistic Bacteria From Spent Mushroom Compost For Controlling Damping-off Cause By Fusarium Solani In Tomato

Aji Zulfikar, Izza Nur Layla, Chaisit Preecha, Wuttichai Seephueak, Pornsil Seephueak


The objective of this study was to test antagonistic bacteria for their ability to control Fusarium solani damping-off disease of tomato (Lycopersicon lycopersicum L.). Antagonistic bacteria were isolated from spent mushroom compost (SMC) of Pleurotus spp. for the pathogenic fungi, Fusarium solani. Thirteen species were tested for the ability to inhibit Fusarium solani by dual culture on PDA plates. Bacillus subtilis subsp. subtilis showed high inhibition of mycelium of Fusarium solani obtained 53.33% until 35 days and then were selected to be further tested in green house conditions. Spore suspension of Fusarium solani (108 spore/ml) were mixed with soil while tomato seeds were soaked in a cell suspension of Bacillus subtilis subsp. subtilis (109 cfu/ml) for 30 min before planting. The experiment used Completely Randomized Design (CRD). The percentages of seed germination and incidence of damping-off compared across 4 treatments; Fusarium solani + Bacillus subtilis subsp. subtilis, Fusarium solani + carbendazim (fungicide), Fusarium solani (disease control) and healthy plant control. The results showed that efficacy was not significantly different. Bacillus subtilis subsp. subtilis effectively controlled the damping-off on tomato by obtained 75% of germination. Whereas, use of Fusarium solani + carbendazim and healthy plant control were obtained 90% and 95%, respectively.

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Adedeji, K.O. and Aduramigba Modupe, A.O. (2016). “In Vitro Evaluation of Spent Mushroom Compost on Growth of Fusarium oxysporum f.sp. lycopersici. Adv. Plant and Agri. Res., 4,


Antoun, H., Prevost, D. (2006). “Ecology of plant growth promoting rhizobacteria., In: PGPR: Biocontrol and Biofertilization. Siddiqui, Z.A. Springer, Dordrecht.

Cawoy, H., Bettiol, W., Fickers, P. and Ongena, M. (2011). “Bacillus-based biological control of plant diseases. In: Stoytcheva, M.(Ed.), Pesticides in the Modern World-Pesticides Use and Management”. InTech, Rijeka, Croatia, pp. 273-302.

Charoenporn, C., Kanokmedhalul, S., Lin, F.C., Poeaim, S., Soytong, K. (2010). “Evaluation of Bio-Agent Formulations to Control Fusarium Wilt to Tomato”. Afi. J. Biotech., 9, 5836-5844.

Cho, S.J., Lee, S.K., Cha, B.J., Kim, Y.H., Shin, K.S. (2003). “Detection and Characterization of the Gloeosporium gloeosporioides Growth Inhibitory Compound Iturin A from Bacillus subtilis Strain KS03”. FEMS Microbiol. Lett., 233, 47-51.

Etchegaray, A., de Castro Bueno, C., de Melo, I.S., Tsai, S.M., de Fatima Fiore, M., Silva Stenico, M.E., de Moraes, L.A.B., Teschke, O. (2008). “Effect of a Highly Concentrated Lipopeptide Extract of Bacillus subtilis on Fungal and Bacterial Cell”. Arch. Microbiol., 190, 611-622.

Fira, D., Dimkić, I., Berić, T., Lozo, J. and Stankovic, S. (2018). “Biological control of plant pathogens by Bacillus species”. J. Biotech., 285, 44-55.

Gbolagade, J.S. (2006). “Bacteria Associated with Compost Used for Cultivation of Nigerian Edible Mushrooms Pleurotus tuber-regium (Fr.) Singer. and Lentinus squarrosulus (Berk.)”. Afri. J. Biotech., 5, 338-342.

Guo, Q., Dong, W., Li, S., Lu, X., Wang, P., Shang, X., Wang, Y., Ma, P. (2014). “Fengycin Produced by Bacillus subtilis NCD-2 Plays Major Role in Biocontrol of Cotton Seedling Damping-Off Disease. Microbiol. Res., 169, 533-540.

Idriss, E.E., Makarewicz, O. Farouk, A, Rosner, K., Greiner, R., Bochow, H., Richter, T., Borriss, R. (2002). “Extracellular Phytase Actibity of Bacillus amyloiquefaciens FZB45 Contributes to Its Plant-Growth-Promoting Effect”. Microbiol., 148, 2097-2109.

Kita, N., Ohya, T., Uekusa, H., Nomura, K., Manago, M., Shoda, M. (2005). “Biological Control of Damping-Off of Tomato Seedlings and Cucumber Phomopsis Root Rot by Bacillus subtilis RB14-C”. JARQ., 39, 109-114.

Kondoh, M., Hirai, M., Shoda, M. (2001). “Integrated Biological and Chemical Control of Damping-off caused by Rhizoctonia solani Using Bacillus subtilis RB14-C and Flutolanil”. J. Biosci. Bioeng., 91, 173-177.

Kondoh, M., Hirai, M., Shoda, M. (2001). “Integrated Biological and Chemical Control of Damping-Off of Tomato Seedlings and Cucumber Phomopsis Root Rot by Bacillus subtilis RB14-C”. JARQ., 39,109-114.

Kumar, V., Singh, P., Jorquera, M.A., Sangwan, P., Kumar, P., Verma, A.K. and Agrawal, S. (2013). “Isolation of Phytase-Producing Bacteria from Himalayan Soils and Their Effect on Growth and Phosphorus Uptake of

Indian Mustard (Brassica juncea)”. World J. Microbiol. Biotechnol., 29, 1361-1369.

Leclère, V., Bechet, M., Adam, A., Guez, J.S., Wathelet, B., Ongena, M., Thonart, P., Gancel, F., Chollet-Imbert, M., Jacques, S. (2005). “Mycosubtilin Overproduction by Bacillus subtilis BBG 100 Enhances the Organism’s Antagonistic and Biocontrol Activities”. Appl. Environ. Microbiol., 71, 4577-4584.

Office of Agricultural Economics. (2018). “Tomato”. tomato.pdf. (accessed 14 September 2018).

Ongena, M., Jacques, P., Toure, Y., Destain, J., Jabrane, A., Thonart, P. (2005). “Involvement of Fengycin-Type Lipopeptides in the Multifaceted Biocontrol Potential of Bacillus subtilis”. App. Microbiol., 69, 29-38.

Recep, K., Fikrettin, S., Erkol, D. and Cafer, E. (2009). “Biological Control of the Potato Dry Rot caused by Fusarium Species Using PGPR Strains”. Biol Control., 50, 194-198.

Reva, O.N., Dixelius, C., Meijer, J., PriestF, G. (2004). “Taxonomic Characterization and Plant Colonizing Abilities of some Bacteria Related to Bacillus amyloliquefaciens, and Bacillus subtilis”. FEMS Microbiol. Ecol., 48, 249-259.

Seephueak, P., Preecha, C. and Seephueak, W. (2016). “Diversity of fungi and bacteria occurring on spent mushroom compost and utilization”. Faculty of Agriculture. Rajamangala University of Technology Srivijaya.

Soytong, K. (1992). “Biological Control of Tomato Wilt caused by Fusarium oxysporum f. sp. lycopersici by using Chaetomium cupreum”. Kasetsart J., 26, 310-313.

Szczech,M. and Shoda, M. (2004). “Biocontrol of Rhizoctonia Damping‐off of Tomato by Bacillus subtilis Combined with Burkholderia cepacia”. J. Phytopath., 152, 549-556.

Thasana, N., Pragagee, B., Rangkadilok, N., Sallabhan, R., Aye, S.L., Ruchirawat, S., Porasert, S., (2010). “Bacillus subtilis SSE4 Produces Subtulene A, a New Lopopeptide Antibiotic Possessing an Unusual C15 Unsaturated B-Amino Acid”. FEBS Lett., 584, 3209-3214.

Watabe, M., Rao, J.R., Xu, J., Miller, B.C., Ward, J.E. and Moore, J.E. (2014). “Identification of Novel Eubacteria from Spent Mushroom Compost (SMC) Waste by DNA Sequence Typing: Ecological Considerations of Disposal on Agricultural Land”. Waste Management., 24, 81-86.

Welbaum, G., Sturz, A.V. Dong, Z. and Nowak, J. (2004). “Fertilizing Soil Microorganisms to Improve Productivity of Agroecosystems”. Crit. Rev. Plant Sci., 23, 175-193.

Zeriouh, H., Romero, D., Garcia-Gutierrez, L., Cazorla, F.M., de Vicente, A., Perez-Garcia, A. (2011). “The Iturin-Like Lipopeptides are Essential Components in the Biological Control Arsenal of Bacillus subtilis against Bacterial Diseases of Cucurbits”. Mol. Plant Microbe. Interact., 24, 1540-1552.

Zhang, N, Wu, K., He, X., Li SQ, Zhang, ZH, Shen, B., Shen, Q.R. (2011). “A New Bioorganic Fertilizer can Effectively Control Banana Wilt by Strong Colonization with Bacillus subtilis N11”. Plant Soil., 344, 87-97.


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